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<title>UTas ePrints - DNA as a dietary biomarker in Antarctic krill, Euphausia superba</title>
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<meta content="Passmore, A.J." name="eprints.creators_name" />
<meta content="Jarman, Simon N." name="eprints.creators_name" />
<meta content="Swadling, Kerrie M." name="eprints.creators_name" />
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<meta content="2007-10-18 02:52:34" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
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<meta content="DNA as a dietary biomarker in Antarctic krill, Euphausia superba" name="eprints.title" />
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<meta content="270702" name="eprints.subjects" />
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<meta content="diet analysis — foodweb — trophic" name="eprints.keywords" />
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<meta content="The diet of Antarctic krill (Euphausia superba) has
been studied using a variety of techniques, but
current methods still suffer from problems that are
difficult to solve. This study examined an alternative
approach utilizing DNA as a prey biomarker.
Methods were developed for the preservation, extraction,
and identification of prey DNA from krill
collected in the field. Group-specific polymerase
chain reaction (PCR) was used to amplify diatom
prey (Phylum: Bacillariophyta) and the results from
DNA clone libraries were compared with microscopic
diet analysis. DNA analysis was superior to
microscopy for prey detection. However, differences
in prey relative abundance estimates between the
two techniques suggested some bias in the DNAbased
estimates. Quantification showed that large
amounts of prey DNA had been successfully preserved
and extracted. Overall the results suggest
that the application of DNA-based diet analysis to
krill warrants further investigation, particularly for
prey that are difficult to study using other methods." name="eprints.abstract" />
<meta content="2006-12" name="eprints.date" />
<meta content="published" name="eprints.date_type" />
<meta content="Marine Biotechnology" name="eprints.publication" />
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<meta content="6" name="eprints.number" />
<meta content="686-696" name="eprints.pagerange" />
<meta content="10.1007/s10126-005-6088-8" name="eprints.id_number" />
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203–214" name="eprints.referencetext" />
<meta content="Passmore, A.J. and Jarman, Simon N. and Swadling, Kerrie M. (2006) DNA as a dietary biomarker in Antarctic krill, Euphausia superba. Marine Biotechnology, 8 (6). pp. 686-696." name="eprints.citation" />
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<meta content="Passmore, A.J." name="DC.creator" />
<meta content="Jarman, Simon N." name="DC.creator" />
<meta content="Swadling, Kerrie M." name="DC.creator" />
<meta content="270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)" name="DC.subject" />
<meta content="The diet of Antarctic krill (Euphausia superba) has
been studied using a variety of techniques, but
current methods still suffer from problems that are
difficult to solve. This study examined an alternative
approach utilizing DNA as a prey biomarker.
Methods were developed for the preservation, extraction,
and identification of prey DNA from krill
collected in the field. Group-specific polymerase
chain reaction (PCR) was used to amplify diatom
prey (Phylum: Bacillariophyta) and the results from
DNA clone libraries were compared with microscopic
diet analysis. DNA analysis was superior to
microscopy for prey detection. However, differences
in prey relative abundance estimates between the
two techniques suggested some bias in the DNAbased
estimates. Quantification showed that large
amounts of prey DNA had been successfully preserved
and extracted. Overall the results suggest
that the application of DNA-based diet analysis to
krill warrants further investigation, particularly for
prey that are difficult to study using other methods." name="DC.description" />
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<h1 class="ep_tm_pagetitle">DNA as a dietary biomarker in Antarctic krill, Euphausia superba</h1>
<p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Passmore, A.J.</span> and <span class="person_name">Jarman, Simon N.</span> and <span class="person_name">Swadling, Kerrie M.</span> (2006) <xhtml:em>DNA as a dietary biomarker in Antarctic krill, Euphausia superba.</xhtml:em> Marine Biotechnology, 8 (6). pp. 686-696.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2162/1/Passmoreetal2006_DNA.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />336Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2703" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1007/s10126-005-6088-8">http://dx.doi.org/10.1007/s10126-005-6088-8</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The diet of Antarctic krill (Euphausia superba) has
been studied using a variety of techniques, but
current methods still suffer from problems that are
difficult to solve. This study examined an alternative
approach utilizing DNA as a prey biomarker.
Methods were developed for the preservation, extraction,
and identification of prey DNA from krill
collected in the field. Group-specific polymerase
chain reaction (PCR) was used to amplify diatom
prey (Phylum: Bacillariophyta) and the results from
DNA clone libraries were compared with microscopic
diet analysis. DNA analysis was superior to
microscopy for prey detection. However, differences
in prey relative abundance estimates between the
two techniques suggested some bias in the DNAbased
estimates. Quantification showed that large
amounts of prey DNA had been successfully preserved
and extracted. Overall the results suggest
that the application of DNA-based diet analysis to
krill warrants further investigation, particularly for
prey that are difficult to study using other methods.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The original publication is available at www.springerlink.com</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">diet analysis — foodweb — trophic</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270702.html">270000 Biological Sciences > 270700 Ecology and Evolution > 270702 Marine and Estuarine Ecology (incl. Marine Ichthyology)</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2162</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Kerrie Swadling</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">18 Oct 2007 13:52</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2162;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=2162">item control page</a></p>
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